cDNA cloning and expression analysis of a mannose-binding lectin from Pinellia pedatisecta

@article{Lin2007cDNACA,
  title={cDNA cloning and expression analysis of a mannose-binding lectin from Pinellia pedatisecta},
  author={J. -L. Lin and Xuanwei Zhou and Shi Gao and Xiaojun Liu and Weisheng Wu and Xiaofen Sun and Kexuan Tang},
  journal={Journal of Biosciences},
  year={2007},
  volume={32},
  pages={241-249}
}
Pinellia pedatisecta agglutinin (PPA) is a very basic protein that accumulates in the tuber of P. pedatisecta. PPA is a hetero-tetramer protein of 40 kDa, composed of two polypeptide chains A (about 12 kDa) and two polypeptides chains B (about 12 kDa). The full-length cDNA of PPA was cloned from P. pedatisecta using SMART RACE-PCR technology; it was 1146 bp and contained a 771 bp open reading frame (ORF) encoding a lectin precursor of 256 amino acid residues with a 24 amino acid signal peptide… CONTINUE READING