cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria.

@article{Yokota1994cDNACA,
  title={cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria.},
  author={Haruo Yokota and Pedro Fern{\'a}ndez-Salguero and Hirokazu Furuya and K Lin and O W Mcbride and B Podschun and Klaus D Schnackerz and Frank J. Gonzalez},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 37},
  pages={23192-6}
}
The pig and human dihydropyrimidine dehydrogenase (DPD) cDNAs were cloned and sequenced. The pig enzyme, expressed in Escherichia coli, catalyzed the reduction of uracil, thymine, and 5-fluorouracil with kinetics approximating those published for the enzyme purified from mammalian liver. DPD could be expressed in significant quantities only when uracil was added to the bacterial growth medium. The pig and human enzymes contained 1025 amino acids and calculated M(r) = 111,416 and 111,398… CONTINUE READING