cDNA cloning and characterization of a 3′/5′-O-methyltransferase for partially methylated flavonols from Chrysosplenium americanum

@article{Gauthier1996cDNACA,
  title={cDNA cloning and characterization of a 3′/5′-O-methyltransferase for partially methylated flavonols from Chrysosplenium americanum},
  author={Antonin Gauthier and Patrick J. Gulick and Ragai K. Ibrahim},
  journal={Plant Molecular Biology},
  year={1996},
  volume={32},
  pages={1163-1169}
}
Enzymatic O-methylation of plant secondary metabolites is an important mechanism for the inactivation of reactive hydroxyl groups and for the modification of their solubility. A cDNA clone (pFOMT3′) encoding the gene for the 3′/5′-O-methylation of partially methylated flavonols was isolated from Chrysosplenium americanum (Saxifragaceae). We used a PCR fragment obtained with degenerate oligonucleotides designed from conserved regions of various O-methyltransferases (OMTs). The pFOMT3′ cDNA… CONTINUE READING