cDNA cloning and assignment to chromosome 21 ofIFI-78K gene, the human equivalent of murineMx gene

  title={cDNA cloning and assignment to chromosome 21 ofIFI-78K gene, the human equivalent of murineMx gene},
  author={Michel Andr{\'e} Horisberger and Marc G. Wathelet and Josiane Szpirer and Claude Szpirer and Q Islam and G{\"o}ran Levan and Georges A. Huez and J. Content},
  journal={Somatic Cell and Molecular Genetics},
Recently we have purified to homogeneity and characterized an interferon-induced human protein (p78 protein) which is the equivalent of the interferon-induced murine Mx protein responsible for a specific antiviral state against influenza virus infection. A cDNA library was constructed using mRNAs from interferon-induced human diploid fibroblasts. cDNA clones coding for the human p78 protein were identified and used to determine the chromosomal location of the corresponding gene (termedIFI-78K… 

Cloning and chromosomal location of human genes inducible by type I interferon

Two cDNA clones corresponding to human genes inducible by IFN-α are isolated by differential screening, termed IFI-4 and Ifi-54K, and it is shown that they are syntenic, unlike most of theIFN-inducible genes.

Four human Chromosome 3q and four human Chromosome 21 loci map onto sheep Chromosome 1q

The in situ localization of transferrin to sheep Chr 1q42-q45 confirms the previous assignment of this locus and independently anchors the eight new syntenic loci to Sheep Chromosome (Chr) 1q.

Understanding KSHV vIRF-2-cell interactions

The role of KSHV vIRF-2 in negative regulation of the IFN-α/β innate immune response by a mechanism dependent on negativeregulation of RIG-I-like receptor, JAK-STAT and Ubiquitin ligase pathways and many gene sets involved in antiviral response, transcriptional regulation and apoptosis are confirmed.

Expression and stability of the Mx protein in different tissues of mice, in response to interferon inducers or to influenza virus infection.

The results indicate that the Mx protein is a sensitive, quantitative, and stable marker to follow IFN activity or virus infection in an animal model.

GART, SON, IFNAR, and CRF2-4 genes cluster on human Chromosome 21 and mouse Chromosome 16

One region of interest includes four genes, GART, SON, IFNAR, and the recently identified CRF2-4, from the chromosome band 21q22, which may contribute to developmental abnormalities of the central nervous system that result from elevated serum purine levels and abnormal adenosine metabolism.

Andes virus stimulates interferon‐inducible MxA protein expression in endothelial cells

The data suggest that MxA by interacting with the virus nucleocapsid protein inhibits production of new infectious virus particles.




It is apparent from these findings that the gene(s) for indophenol oxidase (IPO-B) and the gene for the antiviral protein are syntenic and that they are linked to human chromosome G-21.

erg, a human ets-related gene on chromosome 21: alternative splicing, polyadenylation, and translation.

It is proposed thatAlternative sites of splicing and polyadenylation, together with alternative sites of translation initiation, allow the synthesis of two related polypeptides from a single erg gene transcriptional unit.

Interferon-regulated human 2–5A synthetase gene maps to chromosome 12

The low-molecular-weight human 2–5A synthetase gene has been assigned to chromosome 12 using rodent-human somatic cell hybrids and filter hybridization analysis of cell hybrid DNA. A cDNA probe

Molecular cloning, full-length sequence and preliminary characterization of a 56-kDa protein induced by human interferons.

To understand the molecular basis of the regulation of this protein and of its mRNA, and to understand its biological function and its possible contribution to the various antiviral and non-antiviral actions exerted by interferons, a full-length cDNA copy of the 1.8-1.9 X 10(3)-base 56-kDa-protein mRNA was cloned and its sequence was determined.

Interferon induces a unique protein in mouse cells bearing a gene for resistance to influenza virus.

The results suggest that this protein induced by the interaction ofinterferon with Mx plays a role in the selective antiviral state against influenza viruses that is observed in interferon-treated Mx-bearing cells.

Assignment of the murine interferon sensitivity and cytoplasmic superoxide dismutase genes to chromosome 16.

Both hybrids of mouse and human microcells and whole cell hybrids generated by the fusion of primary mouse cells and SV40-transformed human fibroblasts were used to establish the syntenic association

Human chromosomes 6 and 21 are required for sensitivity to human interferon gamma.

The presence of chromosome 6 in hamster-human hybrids is by itself insufficient to confer sensitivity to human immune interferon as measured by the induction of human HLA, and human chromosome 21 was found to be the second chromosome essential for HLA inducibility.

Assignment of the gene for cytoplasmic superoxide dismutase (Sod-1) to a region of chromosome 16 and of Hprt to a region of the X chromosome in the mouse.

  • U. FranckeR. Taggart
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1979
Using a set of 29 mouse-Chinese hamster somatic cell hybrids, the locus for SOD-1 is assigned to the distal half of mouse chromosome 16 and part of the mouse X chromosome carrying the complementing Hprt gene can be identified by selection in hypoxanthine/aminopterin/thymidine medium and counterselection in 8-azaguanine.

Characterization and Localization of the Human Genes for Ribosomal Ribonucleic Acid

The study of a highly fluorescent chromosome indicates that the ribosomal DNA does not contribute to the fluorescent nature of the secondary constrictions, and the chromosomal inheritance of these genes is explored by in situ hybridization.

Interferon-regulated influenza virus resistance gene Mx is localized on mouse chromosome 16

Genomic Southern blots of mouse-hamster somatic cell hybrids were analyzed with a probe prepared from a cDNA encoding murine Mx protein, the product of the interferon-regulated influenza virus