cDNA cloning, sequence analysis and seasonal expression of lignin-bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase of aspen

@article{Bugos1991cDNACS,
  title={cDNA cloning, sequence analysis and seasonal expression of lignin-bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase of aspen},
  author={Robert C. Bugos and Vincent Lee C. Chiang and Wilbur H. Campbell},
  journal={Plant Molecular Biology},
  year={1991},
  volume={17},
  pages={1203-1215}
}
A cDNA clone (Ptomt1) encoding a lignin-bispecific O-methyltransferase (OMT) was isolated by immunological screening of a λgt11 expression library prepared from mRNA of developing secondary xylem of aspen (Populus tremuloides). Nucleotide sequence analysis of Ptomt1 revealed an open reading frame of 1095 bp which encodes a polypeptide with a predicted molecular weight of 39 802, corresponding well with the size of the OMT polypeptide estimated by SDS-PAGE. Authenticity of Ptomt1 was… CONTINUE READING

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