cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: regulation by environmental CO2 concentration.

@article{Fukuzawa1990cDNACS,
  title={cDNA cloning, sequence, and expression of carbonic anhydrase in Chlamydomonas reinhardtii: regulation by environmental CO2 concentration.},
  author={Hideya Fukuzawa and Shigeyoshi Fujiwara and Yoshio Yamamoto and M L Dionisio-Sese and Shigetoh Miyachi},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 11},
  pages={4383-7}
}
cDNA clones for the periplasmic carbonic anhydrase (CA; carbonate hydro-lyase, EC 4.2.1.1) of Chlamydomonas reinhardtii cells were isolated and characterized. The fact that the cloned cDNA fragments encoded a 377-amino acid polypeptide (41,626 Da) consisting of an NH2-terminal hydrophobic signal peptide of 20 amino acids, a large (35,603 Da) subunit polypeptide, and a small (4144 Da) subunit polypeptide indicates that the two subunits are cotranslated as a precursor polypeptide. The amino acid… CONTINUE READING