cDNA cloning, mRNA expression, and mutational analysis of the squalene synthase gene of Lotus japonicus.

@article{Akamine2003cDNACM,
  title={cDNA cloning, mRNA expression, and mutational analysis of the squalene synthase gene of Lotus japonicus.},
  author={Satomi Akamine and Kazuki Nakamori and Svetlana A. Chechetka and Mari Banba and Yosuke Umehara and Hiroshi Kouchi and K. Izui and Shingo Hata},
  journal={Biochimica et biophysica acta},
  year={2003},
  volume={1626 1-3},
  pages={97-101}
}
A full-length cDNA for squalene synthase was isolated from Lotus japonicus, a model leguminous plant. The transcript was abundant in roots, symbiotic root nodules, and shoots, in that order. In situ hybridization revealed that the mRNA level is high in expanding root cells but low in dividing root tip ones. The transcript is also abundant in vascular bundles and the basal portions of mature nodules. L. japonicus squalene synthase has an unusual Asp residue near the active site, where mammalian… CONTINUE READING