cDNA cloning, homology modelling and evolutionary insights into novel endogenous cellulases of the borer beetle Oncideres albomarginata chamela (Cerambycidae).

@article{CaldernCorts2010cDNACH,
  title={cDNA cloning, homology modelling and evolutionary insights into novel endogenous cellulases of the borer beetle Oncideres albomarginata chamela (Cerambycidae).},
  author={Nancy Calder{\'o}n-Cort{\'e}s and Haruo Watanabe and Horacio Cano-Camacho and Guadalupe Zavala-P{\'a}ramo and M. del Mar Mart{\'i}nez Quesada},
  journal={Insect molecular biology},
  year={2010},
  volume={19 3},
  pages={323-36}
}
Novel endogenous cDNAs of beta-1, 4-endoglucanases (Oa-EGase I and Oa-EGase II) were cloned from the cerambycid beetle Oncideres albomarginata chamela. Oa-EGase I- and Oa-EGase II-deduced proteins and three-dimensional structures possess all features, including general architecture, signature motifs and catalytic domains, of glycosyl hydrolase families 5 and 45 (GHF5 and GHF45) and also share high levels of homology with other beetle cellulases. Total carboxymethylcellulase activity of O. a… CONTINUE READING