c-myc is not involved in cadmium-elicited apoptotic pathway in porcine kidney LLC-PK1 cells.

Abstract

Cadmium chloride can induce DNA fragmentation, a biochemical characteristics of apoptosis in renal epithelial LLC-PK1 cells. Studies of cadmium cytotoxicity demonstrated that cadmium activates c-myc transcription. In this study, we investigated whether c-myc is a necessary component of cadmium-induced apoptosis. By kinetic analysis, transient activation of c-myc transcript by cadmium occurred before DNA fragmentation was induced by the metal, indicating an apparent correlation between induction of c-myc mRNA and promotion of apoptosis. However, even when using actinomycin D to block transcriptional activation of c-myc, or antisense oligodeoxynucleotide complementary to c-myc to block translation of c-myc, cadmium could still induce apoptosis. Thus, our data show that cadmium elicits apoptosis by a mechanism other than regulation of c-myc expression: transcriptional activation of c-myc during apoptosis is not always involved in the cell-death events.

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@article{Ishido1998cmycIN, title={c-myc is not involved in cadmium-elicited apoptotic pathway in porcine kidney LLC-PK1 cells.}, author={Masami Ishido and Chiharu Tohyama and T Suzuki}, journal={Life sciences}, year={1998}, volume={63 14}, pages={1195-204} }