c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility


Individual cell heterogeneity is commonly observed within populations, although its molecular basis is largely unknown. Previously, using FRET-based microscopy, we observed heterogeneity in cellular c-di-GMP levels. In this study, we show that c-di-GMP heterogeneity in Pseudomonas aeruginosa is promoted by a specific phosphodiesterase partitioned after cell division. We found that subcellular localization and reduction of c-di-GMP levels by this phosphodiesterase is dependent on the histidine kinase component of the chemotaxis machinery, CheA, and its phosphorylation state. Therefore, individual cell heterogeneity in c-di-GMP concentrations is regulated by the activity and the asymmetrical inheritance of the chemotaxis organelle after cell division. c-di-GMP heterogeneity results in a diversity of motility behaviors. The generation of diverse intracellular concentrations of c-di-GMP by asymmetric partitioning is likely important to the success and survival of bacterial populations within the environment by allowing a variety of motility behaviors. DOI: http://dx.doi.org/10.7554/eLife.01402.001.

DOI: 10.7554/eLife.01402

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@inproceedings{Kulasekara2013cdiGMPHI, title={c-di-GMP heterogeneity is generated by the chemotaxis machinery to regulate flagellar motility}, author={Bridget R. Kulasekara and Cassandra N Kamischke and Hemantha Don Kulasekara and Matthias Christen and Paul A. Wiggins and Samuel I. Miller}, booktitle={eLife}, year={2013} }