bldA dependence of undecylprodigiosin production in Streptomyces coelicolor A3(2) involves a pathway-specific regulatory cascade

@article{White1997bldADO,
  title={bldA dependence of undecylprodigiosin production in Streptomyces coelicolor A3(2) involves a pathway-specific regulatory cascade},
  author={J. White and M. Bibb},
  journal={Journal of Bacteriology},
  year={1997},
  volume={179},
  pages={627 - 633}
}
The production of the red-pigmented tripyrrole antibiotic undecylprodigiosin (Red) by Streptomyces coelicolor A3(2) depends on two pathway-specific regulatory genes, redD and redZ. RedD is homologous to several other proteins that regulate antibiotic production in streptomycetes; RedZ is a member of the response regulator family. redZ transcripts were detected during exponential growth and increased in amount during transition and stationary phases; transcription of redD was confined to the two… Expand
Application of redD, the transcriptional activator gene of the undecylprodigiosin biosynthetic pathway, as a reporter for transcriptional activity in Streptomyces coelicolor A3(2) and Streptomyces lividans.
TLDR
The ability to screen visually and spectrophotometrically for red pigmentation should make the vectors particularly attractive for analysing the regulation of gene expression, and for the isolation of mutants, in both S. coelicolor and S. lividans. Expand
Characterization of rrdA, a TetR Family Protein Gene Involved in the Regulation of Secondary Metabolism in Streptomyces coelicolor
TLDR
An in vivo transposition system was used to identify novel regulators that influence Red production in Streptomyces coelicolor M145 and showed that RrdA negatively regulated Red production by controlling redD mRNA abundance, while no change was observed at the transcript level of the Act-specific activator gene, actII-orf4. Expand
A response-regulator-like activator of antibiotic synthesis from Streptomyces coelicolor A3(2) with an amino-terminal domain that lacks a phosphorylation pocket.
In Streptomyces coelicolor A3(2), bldA mutants that lack the tRNA for the rare leucine codon UUA fail to make the red undecylprodigiosin antibiotic complex. To find out why, red-pigmented while baldExpand
Activation of Antibiotic Biosynthesis by Specified Mutations in the rpoB Gene (Encoding the RNA Polymerase β Subunit) of Streptomyces lividans
TLDR
Results indicate that the mutation in the rpoB gene of S. lividans, resulting in the activation of Act and/or Red biosynthesis, functions at the transcription level by activating directly or indirectly the key regulatory genes, actII-ORF4 and redD. Expand
Different effects of acidic pH shock on the prodiginine production in Streptomyces coelicolor M511 and SJM1 mutants.
TLDR
RT-PCR analyses showed that, during pH shock, expression of redD, the transcription activator gene, was increased while the expression of fabH, the decarboxylative condensation enzyme gene in fatty acid biosynthesis, was decreased in both strains. Expand
Characterization of regulatory pathways controlling morphological differentiation in "Streptomyces coelicolor"
TLDR
DNA array analyses suggest the identity of more than 300 genes belonging to the ramR regulon, leading to the discovery of a locus of cotranscribed developmental genes that has a role in both AHF and sporulation and demonstrated that SapB can trigger the activation of a morpgohenetic program to the emerging aerial hyphae. Expand
Morphological differentiation and clavulanic acid formation are affected in a Streptomyces clavuligerus adpA-deleted mutant.
TLDR
Quantitative transcriptional analysis indicates that expression of the clavulanic acid regulatory genes ccaR and claR decreases seven- and fourfold, respectively, in the DeltaadpA mutant, resulting in a large decrease in expression of genes encoding biosynthesis enzymes for the early steps of clavULanic acid formation and a smaller decrease in the expression of gene for the late steps of the pathway. Expand
The positive activator of cephamycin C and clavulanic acid production in Streptomyces clavuligerus is mistranslated in a bldA mutant.
In Streptomyces coelicolor bldA encodes the principal leucyl tRNA for translation of UUA codons and controls pigmented antibiotic production by the presence of TTA codons in the genes encoding theExpand
Altered desferrioxamine-mediated iron utilization is a common trait of bald mutants of Streptomyces coelicolor.
TLDR
This study allows proposing altered DFO production and/or FO uptake as a novel phenotypic marker of many S. coelicolor bld mutants, and strengthens the role of siderophores and iron acquisition in morphological development of actinomycetes. Expand
afsQ1-Q2-sigQ is a pleiotropic but conditionally required signal transduction system for both secondary metabolism and morphological development in Streptomyces coelicolor
TLDR
It is found that deletion of sigQ, which is located upstream of afsQ1-Q2 and encodes a putative sigma factor, led to the precocious hyperproduction of these antibiotics and delayed formation of sporulating aerial mycelium in the same glutamate-based defined MM. Expand
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References

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Nucleotide sequence and transcriptional analysis of the redD locus of Streptomyces coelicolor A3(2)
TLDR
The putative redD polypeptide lacked any strong sequence similarities to other known proteins and was not involved in the regulation of undecylprodigiosin (Red) biosynthesis in Streptomyces coelicolor. Expand
Transcriptional regulation of the redD transcriptional activator gene accounts for growth‐phase‐dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2)
TLDR
Transcription of redD, the activator gene required for production of the red‐pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase, which appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters. Expand
afsR is a pleiotropic but conditionally required regulatory gene for antibiotic production in Streptomyces coelicolor A3(2)
TLDR
It is demonstrated that multiple copies of afsR can stimulate both Act and Red production and that, despite its homology, it cannot substitute for the pathway‐specific regulatory genes. Expand
Stationary‐phase production of the antibiotic actinorhodin in Streptomyces coelicolor A3(2) is transcriptionally regulated
TLDR
Production of actinorhodin, a polyketide antibiotic made by Streptomyces coelicolor A3(2), normally occurs only in stationary‐phase cultures, but normal growth‐phase‐dependent production in liquid culture conditions appears to be mediated at the transcriptional level through activation of the actII‐ORF4 promoter. Expand
Genetic and biochemical characterization of the red gene cluster of Streptomyces coelicolor A3(2).
TLDR
Experiments in liquid antibiotic production medium indicated that prodigiosin compounds in S. coelicolor are examples of 'secondary metabolites' whose synthesis lags behind that of cell mass, and perhaps to 'secondary' metabolism in general. Expand
Additional copies of the actII regulatory gene induce actinorhodin production in pleiotropic bld mutants of Streptomyces coelicolor A3(2)
TLDR
The results indicate that the structural genes for actinorhodin biosynthetic enzymes do not contain targets for the bldA, D, F, G and H gene products, and suggest that the corresponding bld genes control actin orhodin synthesis principally via a single gene in the actII regulatory region. Expand
Regulation of daunorubicin production in Streptomyces peucetius by the dnrR2 locus
Sequence analysis of the dnrR2 locus from the cluster of daunorubicin biosynthesis genes in Streptomyces peucetius ATCC 29050 has revealed the presence of two divergently transcribed open readingExpand
The level of a transcript required for production of a Streptomyces coelicolor antibiotic is conditionally dependent on a tRNA gene
TLDR
These experiments showed that the undecylprodigiosin deficiency of bldA mutants cannot be accounted for by the presence of TTA codons in the red structural genes, but rather that bld a influences red gene mRNA abundance. Expand
Identification of a red pigment from Streptomyces coelicolor A3(2) as a mixture of prodigiosin derivatives.
TLDR
On the chemical nature of the red pigment is reported, which is blocked in the synthesis of the diffusible pigment actinorhodin3 by UV irradiation of strain B18. Expand
Regulation of secondary metabolism in Streptomyces spp. and overproduction of daunorubicin in Streptomyces peucetius
TLDR
Two DNA segments from the Streptomyces peucetius ATCC 29050 genome were identified by their ability to stimulate secondary metabolite production and resistance and are likely to be trans-acting factors involved in regulating secondary metabolism. Expand
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