beta-Galactosidase activity assay using far-red-shifted fluorescent substrate DDAOG.

@article{Gong2009betaGalactosidaseAA,
  title={beta-Galactosidase activity assay using far-red-shifted fluorescent substrate DDAOG.},
  author={Haibiao Gong and Bin Zhang and Garrick M. Little and Joy Kovar and Huaxian Chen and Wen Yuan Xie and Amy Schutz-Geschwender and David Michael Olive},
  journal={Analytical biochemistry},
  year={2009},
  volume={386 1},
  pages={
          59-64
        }
}
beta-Galactosidase (beta-gal) is commonly used as a reporter gene in biological research, and a wide variety of substrates have been developed to assay its activity. One substrate, 9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) beta-d-galactopyranoside (DDAOG), can be cleaved by beta-gal to produce 7-hydroxy-9H(I,3-dichloro-9,9-dimethylacridin-2-one) (DDAO). On excitation, DDAO generates a far-red-shifted fluorescent signal. Using this substrate, we developed a beta-gal activity assay method… CONTINUE READING
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