beta-Amyloid increases enzyme activity and protein levels of glutamine synthetase in cultured astrocytes.

Abstract

Previous studies have reported that beta-amyloid peptides induce properties of reactivity in cultured astrocytes. We report here that aggregated A beta peptides increase expression of the enzyme glutamine synthetase in cultured astrocytes, as assessed by enzyme assay, Western blot analysis, and immunocytochemistry. The enhanced enzyme levels occur gradually over a period of 4 days after A beta exposure and maintain peak values for at least several days thereafter. These data suggest that A beta-related reactive astrocytosis in Alzheimer's disease brain may benefit local neurons by enhancing glial capacity to regulate levels of the excitotoxin glutamate.

Statistics

0100200'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

183 Citations

Semantic Scholar estimates that this publication has 183 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Pike1996betaAmyloidIE, title={beta-Amyloid increases enzyme activity and protein levels of glutamine synthetase in cultured astrocytes.}, author={Christian J Pike and Nima Ramezan-Arab and S Miller and Carl Cotman}, journal={Experimental neurology}, year={1996}, volume={139 1}, pages={167-71} }