alpha-Bungarotoxin immobilized and oriented on a lipid bilayer vesicle surface.

We have developed a new method to assess the binding site on alpha-bungarotoxin (alpha-BGT) for the acetylcholine receptor. It involves the covalent attachment of a palmitic acid chain to the toxin molecule, generating monopalmitoyl-alpha-bungarotoxin (PBGT) which is then immobilized on the surface of a lipid vesicle by a process of spontaneous insertion… CONTINUE READING