alpha-Amylase is not required for breakdown of transitory starch in Arabidopsis leaves.

@article{Yu2005alphaAmylaseIN,
  title={alpha-Amylase is not required for breakdown of transitory starch in Arabidopsis leaves.},
  author={Tien-Shin Yu and Samuel C Zeeman and David Thorneycroft and Daniel C. Fulton and Hannah Dunstan and Wei-ling L{\"u} and Bj{\"o}rn Hegemann and Shu-Yun Tung and Takayuki Umemoto and Andrew Chapple and Der-Long Tsai and Shue-Mei Wang and Alison M Smith and Jychian Chen and Steven M Smith},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 11},
  pages={9773-9}
}
The Arabidopsis thaliana genome encodes three alpha-amylase-like proteins (AtAMY1, AtAMY2, and AtAMY3). Only AtAMY3 has a predicted N-terminal transit peptide for plastidial localization. AtAMY3 is an unusually large alpha-amylase (93.5 kDa) with the C-terminal half showing similarity to other known alpha-amylases. When expressed in Escherichia coli, both the whole AtAMY3 protein and the C-terminal half alone show alpha-amylase activity. We show that AtAMY3 is localized in chloroplasts. The… CONTINUE READING