the loss of WGA lectin receptors in human corneal epithelium. Use of cryosections may be preferable, especially when a negative reaction is obtained using paraffin sections. It has been shown that human corneal epithelium stains with Lotus tetragonolobus and Dolichos biflorus only if frozen sections are used. In recent years, paraffin sections have been used extensively with success in the study of lectin binding sites. Formalin fixation and paraffin embedding would not be expected to alter lectin binding sites chemically, because glycosidic bonds are stable at high temperatures (up to 100°C) and in organic solvents as long as pH is maintained close to 7.0. Differences in the lectin staining patterns of frozen and paraffin sections are probably related to differences in the solubility properties of various macromolecules. Lipid-like molecules are extracted in solvents used for deparaffinization, and are thus not expected to be retained. On the other hand, glycoproteins are often retained in the formalin-fixed paraffin sections.