Zone broadening in electrophoresis with special reference to high-performance electrophoresis in capillaries: an interplay between theory and practice.

@article{Hjertn1990ZoneBI,
  title={Zone broadening in electrophoresis with special reference to high-performance electrophoresis in capillaries: an interplay between theory and practice.},
  author={Stellan Hjert{\'e}n},
  journal={Electrophoresis},
  year={1990},
  volume={11 9},
  pages={665-90}
}
Approximate equations have been derived for the total (final) zone width (plate height, plate number and resolution) as a function of the width of the starting zone and of the zone broadening caused by diffusion. Joule heat, adsorption and the difference in conductivity (delta kappa) between a solute zone and the surrounding buffer. Two cases are treated… CONTINUE READING