Zinc metalloprotease activity in the cement precursor secretion of the barnacle, Chthamalus fragilis Darwin.

@article{Dougherty1996ZincMA,
  title={Zinc metalloprotease activity in the cement precursor secretion of the barnacle, Chthamalus fragilis Darwin.},
  author={William J. Dougherty},
  journal={Tissue & cell},
  year={1996},
  volume={28 4},
  pages={
          439-47
        }
}
Adult barnacles, Chathamalus fragilis, were removed carefully from the leaves and stems of marsh grass and floated base-up in algae-supplemented sea water. During the next 24-72 h, the animals secreted onto their exposed bases, a fluid, the cement precursor secretion (CPS), aliquots of which were collected in glass micropipets and pooled. The concentration of protein in pooled samples of CPS averaged 1.5 g +/- 0.42 protein/l of secretion. Protease activity was expressed as A(492) units/h/g of… CONTINUE READING