Zinc ions stimulate the cooperative RNA binding of hordeiviral γb protein

@inproceedings{Rakitina2006ZincIS,
  title={Zinc ions stimulate the cooperative RNA binding of hordeiviral γb protein},
  author={Daria V. Rakitina and Natalia E. Yelina and Natalia O. Kalinina},
  year={2006}
}
A small regulatory γb protein of the Poa semilatent hordeivirus (PSLV) contains two zinc finger-like motifs separated by a basic motif in the N-terminal part and a C-terminal coiled-coil motif. Interactions of the recombinant PSLV γb protein and its mutants with various RNAs (ssRNA, dsRNA, ssRNA oligonucleotides) and ssDNA were studied in gel-shift assays. The results demonstrated that zinc ions are essential for effective nucleic-acid-binding activity of the γb protein, suggesting the… CONTINUE READING