Zinc-human serum albumin association: testimony of two binding sites.

@article{Andr2004ZinchumanSA,
  title={Zinc-human serum albumin association: testimony of two binding sites.},
  author={Claire Andr{\'e} and Yves Claude Guillaume},
  journal={Talanta},
  year={2004},
  volume={63 2},
  pages={
          503-8
        }
}
The zinc cation (Zn(2+)) binding to human serum albumin (HSA) was studied using a non-equilibrium approach in order to prove two HSA binding sites. The effect of the bulk solvent pH and column temperature T on this binding and the corresponding thermodynamic data were also investigated. It appeared that the association process can be divided into two pH value ranges due to a predominant Zn(2+) interaction with either HSA site I or site II. It was also demonstrated that the Zn(2+) affinity for… CONTINUE READING
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