Zinc-finger recombinase activities in vitro

  title={Zinc-finger recombinase activities in vitro},
  author={Marko M. Prorocic and Dong Wenlong and Femi J. Olorunniji and Aram Akopian and Jan-Gero Schloetel and Ad{\`e}le Hannigan and Arlene L. McPherson and W. Marshall Stark},
  booktitle={Nucleic acids research},
Zinc-finger recombinases (ZFRs) are chimaeric proteins comprising a serine recombinase catalytic domain linked to a zinc-finger DNA binding domain. ZFRs can be tailored to promote site-specific recombination at diverse 'Z-sites', which each comprise a central core sequence flanked by zinc-finger domain-binding motifs. Here, we show that purified ZFRs catalyse efficient high-specificity reciprocal recombination between pairs of Z-sites in vitro. No off-site activity was detected. Under different… CONTINUE READING
Recent Discussions
This paper has been referenced on Twitter 1 time over the past 90 days. VIEW TWEETS

From This Paper

Topics from this paper.
14 Citations
35 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 14 extracted citations


Publications referenced by this paper.
Showing 1-10 of 35 references

Similar Papers

Loading similar papers…