Zinc-finger nucleases

@article{Souza2011ZincfingerN,
  title={Zinc-finger nucleases},
  author={Natalie de Souza},
  journal={Nature Methods},
  year={2011},
  volume={8},
  pages={43-43}
}
Based on rapid methodological developments in 2009, last year we selected targeted proteomics analysis by mass spectrometry as a Method to Watch. Progress in 2010 continued at a quickening pace. The targeted proteomics approach differs fundamentally from the more familiar ‘shotgun’ approach in which the spectra generated from all detectable proteins in a sample are interpreted by database searching. In a targeted analysis, the mass spectrometer is programmed to analyze a preselected group of… 
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Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures
TLDR
The development and application of a yeast-based selection system designed to functionally interrogate the ZFN dimer interface is reported, identified critical residues involved in dimerization through the isolation of cold-sensitive nuclease domains, and used to engineer ZFNs that have superior cleavage activity while suppressing homodimerization.
Selection-Free Zinc-Finger Nuclease Engineering by Context-Dependent Assembly (CoDA)
TLDR
Context-dependent assembly (CoDA) is described, a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis and rapidly altered 20 genes in Danio rerio, Arabidopsis thaliana and Glycine max.