Zinc-finger intron 7: a new locus for sex identification of giant panda (Ailuropoda melanoleuca).

  title={Zinc-finger intron 7: a new locus for sex identification of giant panda (Ailuropoda melanoleuca).},
  author={Xiao Xu and Yuzhi Li and Xiaofang Wang and Kun Wei and Wenping Zhang and Zhihe Zhang and Fujun Shen and Bisong Yue},
  journal={Zoo biology},
  volume={29 4},
We developed a single-reaction test for identifying the sex of giant panda (Ailuropoda melanoleuca) targeted to co-amplify homologous fragments with size polymorphism that located at zinc-finger (ZF) intron 7 by using one pair of primers. This assay produced one sex-specific fragment in females (XX genotypes) whereas two fragments were produced in males (XY genotypes). Indels (insertion/deletion) in intron 7 of Y-linked allele provide a significant discrimination between ZFX and ZFY, thus the… CONTINUE READING

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