Zinc finger-based knockout punches for zebrafish genes.

@article{Ekker2008ZincFK,
  title={Zinc finger-based knockout punches for zebrafish genes.},
  author={Stephen C. Ekker},
  journal={Zebrafish},
  year={2008},
  volume={5 2},
  pages={121-3}
}
The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental… CONTINUE READING