Zinc finger-based knockout punches for zebrafish genes.

  title={Zinc finger-based knockout punches for zebrafish genes.},
  author={Stephen C. Ekker},
  volume={5 2},
The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental… CONTINUE READING