Dermatophytosis (ringworm), an infection of the superficial keratinized structures of the skin and hair, is the most common contagious skin disease in cattle. The infectious diseases caused by dermatophytes are mainly related to the enzymes product by these fungi. Conversely, elements such as zinc and selenium are involved in the regulation of immune responses to infection. There are rare reports about the possible role of zinc and selenium concentration in the pathogenesis of cattle dermatophytosis. Thus, this study was conducted in a humid area of Iran on 35 healthy and 35 infected cows. After diagnosis confirmation by direct microscopic examination and fungi isolation via inoculation on Sabouraud dextrose agar using skin scrap and broken hair samples of infected cows, the zinc and selenium concentration of serum and hair in both groups were determined by potentiometric stripping analyzer and atomic absorption spectrometry, respectively. Results showed that serum concentration of selenium and zinc in cattle with dermatophytosis were significantly lower (P < 0.05) than the healthy ones. Although hair concentration of selenium and zinc in infected cattle were lower than the healthy ones, the differences were not significant (P > 0.05). In conclusion, it seems that zinc and selenium have a determinant role in immune status and the response of animal’s immunity system to dermatophytosis.