Zeptomole-sensitivity electrospray ionization--Fourier transform ion cyclotron resonance mass spectrometry of proteins.

Abstract

Methods are being developed for ultrasensitive protein characterization based upon electrospray ionization (ESI) with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). The sensitivity of a FTICR mass spectrometer equipped with an ESI source depends on the overall ion transmission, which combines the probability of ionization, transmission efficiency, and ion trapping in the FTICR cell. Our developments implemented in a 3.5 tesla FTICR mass spectrometer include introduction and optimization of a newly designed electrodynamic ion funnel in the ESI interface, improving the ion beam characteristics in a quadrupole-electrostatic ion guide interface, and modification of the electrostatic ion guide. These developments provide a detection limit of approximately 30 zmol (approximately 18,000 molecules) for proteins with molecular weights ranging from 8 to 20 kDa.

Statistics

050100'02'04'06'08'10'12'14'16
Citations per Year

164 Citations

Semantic Scholar estimates that this publication has 164 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Belov2000ZeptomolesensitivityEI, title={Zeptomole-sensitivity electrospray ionization--Fourier transform ion cyclotron resonance mass spectrometry of proteins.}, author={Mikhail E. Belov and Mikhail V Gorshkov and Harold R. Udseth and Gordon A. Anderson and Richard D. Smith}, journal={Analytical chemistry}, year={2000}, volume={72 10}, pages={2271-9} }