Zein gene organization in maize and related grasses

  title={Zein gene organization in maize and related grasses},
  author={Deborah R. Wilson and Brian A. Larkins},
  journal={Journal of Molecular Evolution},
Zein cDNA clones were used to study the organization of zein genes within the genome of the inbred maize W64A. When individual clones for the two larger molecular-weight classes of zein proteins (Mr=22,000; Mr=19,000) were used as probes for Southern blot hybridizations of genomic DNA, multiple restriction fragments were found to hybridize. Reconstruction analyses using moderately stringent criteria were used to estimate a total of 70–80 zein sequences within the genome of this inbred maize… CONTINUE READING