Zebrafish nodal-related genes are implicated in axial patterning and establishing left-right asymmetry.

@article{Rebagliati1998ZebrafishNG,
  title={Zebrafish nodal-related genes are implicated in axial patterning and establishing left-right asymmetry.},
  author={Michael R. Rebagliati and Reiko Toyama and Cornelia Fricke and Pascal Haffter and Igor B Dawid},
  journal={Developmental biology},
  year={1998},
  volume={199 2},
  pages={
          261-72
        }
}
Nodal-related 1 (ndr1) and nodal-related 2 (ndr2) genes in zebrafish encode members of the nodal subgroup of the transforming growth factor-beta superfamily. We report the expression patterns and functional characteristics of these factors, implicating them in the establishment of dorsal-ventral polarity and left-right asymmetry. Ndr1 is expressed maternally, and ndr1 and ndr2 are expressed during blastula stage in the blastoderm margin. During gastrulation, ndr expression subdivides the shield… 
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  • Biology
    Philosophical Transactions of the Royal Society B: Biological Sciences
  • 2016
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An unexpected role for the zebrafish EGF-CFC gene one-eyed pinhead (oep) in the midline to promote pitx2 expression in the LPM is reported, which suggests a model where Oep in theMidline functions to overcome a midline-derived repressor, involving lefty1, to allow for the expression of left side-specific genes in theLPM.
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The data demonstrates that cells respond to Nodal-related proteins by a novel time-dependent mechanism that is not yet fully characterized, and shows that NODal signals from the extraembryonic tissues are required to pattern embryonic tissues.
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References

SHOWING 1-10 OF 88 REFERENCES
Functional differences among Xenopus nodal-related genes in left-right axis determination.
TLDR
These findings suggest that the various Xnrs perform substantially different functions during Xenopus embryogenesis, and strongly support the hypothesis that left lateral plate expression of nodal-related genes is a causative factor in the determination of asymmetry in vertebrate embryos.
Relationship between asymmetric nodal expression and the direction of embryonic turning
TLDR
It is observed in inv mutant embryos8 that the sidedness of nodal expression correlates with the direction of heart looping and embryonic turning, demonstrating that elements of the genetic pathway that establish the left–right body axis are conserved in vertebrates.
Nodal-related signals induce axial mesoderm and dorsalize mesoderm during gastrulation.
TLDR
Two Xenopus nodal-related genes (Xnr-1 and Xnr-2) are identified and shown to be expressed transiently during embryogenesis, first within the vegetal region of late blastulae and later in the marginal zone during gastrulation, with enrichment in the dorsal lip.
Nodal induces ectopic goosecoid and lim1 expression and axis duplication in zebrafish.
TLDR
A role for a nodal-like factor in pattern formation in zebrafish is suggested, as indicated by the appearance of a region over-expressing gsc and lim1 and isolation and expression in the shield of the lim1 gene.
A Xenopus nodal-related gene that acts in synergy with noggin to induce complete secondary axis and notochord formation.
TLDR
Results suggest that the nodal-related gene may act together with noggin to induce axial pattern during gastrulation and also may play a role in left-right asymmetry generation in the post-gastrula embryo.
A nodal-related gene defines a physical and functional domain within the Spemann organizer
Genes establishing dorsoventral pattern formation in the zebrafish embryo: the ventral specifying genes.
TLDR
The similar and overlapping phenotypes of mutants of the 6 genes identified suggest that they function in a common pathway, which begins in oogenesis, but also depends on factors provided after the onset of zygotic transcription, presumably during blastula stages.
The ventral and posterior expression of the zebrafish homeobox gene eve1 is perturbed in dorsalized and mutant embryos.
TLDR
It is shown that LiCl, known to dorsalize Xenopus embryos, has the same effect in zebrafish, resulting in embryos with exaggerated dorsoanterior structures, and first insights into its regulation are provided.
Nodal is a novel TGF-β-like gene expressed in the mouse node during gastrulation
TLDR
The findings suggest that this gene, named nodal, encodes a signalling molecule essential for mesoderm formation and subsequent organization of axial structures in early mouse development.
A molecular pathway determining left-right asymmetry in chick embryogenesis
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