Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization

  title={Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization},
  author={Tim Brend and Scott A. Holley},
  booktitle={Journal of visualized experiments : JoVE},
Whole mount in situ hybridization is one of the most widely used techniques in developmental biology. Here, we present a high-resolution double fluorescent in situ hybridization protocol for analyzing the precise expression pattern of a single gene and for determining the overlap of the expression domains of two genes. The protocol is a modified version of the standard in situ hybridization using alkaline phosphatase and substrates such as NBT/BCIP and Fast Red (1,2). This protocol utilizes… CONTINUE READING


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