Zebrafish (Danio rerio) and Japanese Medaka (Oryzias latipes) as Model Species for Evaluation of Endocrine Disrupting Chemicals

Abstract

1 BACKGROUND 10 1.1 Endocrine disrupting chemicals (EDCs) 10 1.2 Deleterious effects of EDCs in vertebrates 10 1.3 Mechanism of endocrine disruption 11 1.4 Steroid 12 1.4.1 Estrogens 12 1.4.2 Androgens 14 1.5 Impact of EDCs on fish 15 1.6 Fish models for evaluation of EDCs 16 1.6.1 Zebrafish (Danio rerio) 16 1.6.2 Japanese Medaka (Oryzias latipes) 17 1.6.3 Fathead minnow (Pimephales promelas) 17 1.7 Fish life cycle assays 17 1.7.1 Fish screening assay 17 1.7.2 42-days Reproductive Fitness Test 18 1.7.3 Fish Sexual Develomental TestComplete Life Cycle Test / fish-full-life-cycle-test 18 1.8 Biomarkers 18 1.8.1 Vitellogenin 18 1.8.2 Fish gonads & histology 20 1.8.2.1 Ovary 20 1.8.2.2 Testis 21 2 GENERAL AIM OF THE THESIS 22 3 MAJOR CONCLUSIONS 22 4 REFERENCES 23 5 RESEARCH PAPER I 30 17 -ethinylestradiol (EE2) induced vitellogenin levels and normal gonad morphology during early stages of life cycle in Japanese medaka (Oryzias latipes) 6 RESEARCH PAPER II 41 Comparison of vitellogenin induction and gonad morphology between zebrafish and Japanese medaka after exposure to 17 -ethinylestradiol and 17 -trenbolone 7 ACKNOWLEDGEMENTS 56

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Cite this paper

@inproceedings{Yamani2004ZebrafishR, title={Zebrafish (Danio rerio) and Japanese Medaka (Oryzias latipes) as Model Species for Evaluation of Endocrine Disrupting Chemicals}, author={Suresh Yamani and Benjam{\'i}n Franklin}, year={2004} }