Zebrafish: model for the study of inflammation and the innate immune response to infectious diseases.

  title={Zebrafish: model for the study of inflammation and the innate immune response to infectious diseases.},
  author={Beatriz Novoa and Antonio Figueras},
  journal={Advances in experimental medicine and biology},
The zebrafish (Danio rerio) has been extensively used in biomedical research as a model to study vertebrate development and hematopoiesis and recently, it has been adopted into varied fields including immunology. After fertilization, larvae survive with only the innate immune responses because adaptive immune system is morphologically and functionally mature only after 4-6 weeks postfertilization. This temporal separation provides a suitable system to study the vertebrate innate immune response… 
Infectious disease models in zebrafish.
Conserved gene regulation during acute inflammation between zebrafish and mammals
It is formally confirmed that zebrafish inflammatory models are suited to study the basic mechanisms of inflammation in human inflammatory diseases, with great translational impact potential.
Tipping the Scales With Zebrafish to Understand Adaptive Tumor Immunity
An overview of the development of the zebrafish immune system is provided along with a side-by-side comparison of its human counterpart and components of the adaptive immune system with a focus on their roles in the tumor microenvironment of teleosts are introduced.
Establishment of Infection Models in Zebrafish Larvae (Danio rerio) to Study the Pathogenesis of Aeromonas hydrophila
The suitability of zebrafish larvae to study the pathogenesis of A. hydrophila and the interaction mechanisms between the bacterium and the innate immune responses through an infection model using different routes for infection is explored.
Chapter 2 Animal Models of Tuberculosis : Zebrafish
Over the past decade the zebrafish (Danio rerio) has become an attractive new vertebrate model organism for studying mycobacterial pathogenesis. The combination of mediumthroughput screening and
Studying the immune response to human viral infections using zebrafish.
Organ-specific LPS-induced inflammatory gene expression in adult Zebrafish
A lipopolysaccharide (LPS) model of systemic inflammation in vivo using zebrafish was generated to identify organspecific proinflammatory genes activity after intraperitoneal LPS infusion and demonstrated that the investigation of isolated zebra fish organs could be useful and informative for the Investigation of inflammatory processes.
Zebrafish: modeling for herpes simplex virus infections.
A fully developed immune system and potential for receptor-specific knockouts further advocate the use of zebrafish as a new tool to study viral infections.


Zebrafish as a model for infectious disease and immune function.
In vivo analysis of zebrafish innate immunity.
This chapter provides a protocol for the generation of zebrafish embryos deficient in a protein of interest for innate immune signaling using antisense morpholino oligonucleotides, the systemic or local infection of these embryos with bacteria, and the assessment of various aspects of the following immune response with emphasis on microscopic observation.
Using in vivo zebrafish models to understand the biochemical basis of neutrophilic respiratory disease.
This model has visualized the process of inflammation resolution in vivo, and identified a role for apoptosis in this process, and established a platform for screening for compounds with anti-inflammatory activity, which has yielded a number of interesting leads.
Transcriptome Profiling and Functional Analyses of the Zebrafish Embryonic Innate Immune Response to Salmonella Infection1
This study performed a time-course transcriptome profiling study and gene ontology analysis of the embryonic innate immune response to infection with two model Salmonella strains that elicit either a lethal infection or an attenuated response, leading to the identification of a large set of novel immune response genes and infection markers.
The zebrafish as a model organism to study development of the immune system.
Streptococcus-Zebrafish Model of Bacterial Pathogenesis
The genetic systems available for streptococci, including a novel method of mutagenesis which targets genes whose products are exported, were used to identify several mutants attenuated for virulence in zebrafish, making the Streptococcus-zebrafish model of bacterial pathogenesis a powerful model for analysis of infectious disease.
Host Immune Response and Acute Disease in a Zebrafish Model of Francisella Pathogenesis
The pathology and immune response to acute Francisella infection in zebrafish share many features with those in mammals, highlighting the usefulness of this new model system for addressing both general and specific questions about Francisella host-pathogen interactions via an evolutionary approach.
Characterization of rag1 mutant zebrafish leukocytes
In comparison to wild-type zebrafish, rag 1 mutants have a significantly reduced lymphocyte-like cell population that likely includes Non-specific cytotoxic cells (NCC) and NK cells (and lacks functional T and B lymphocytes), a similar macrophage/monocyte population, and a significantly increased neutrophil population.
Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota.
Methods for producing and rearing germ-free zebrafish through late juvenile stages are developed and established as a useful model for dissecting the molecular foundations of host-microbial interactions in the vertebrate digestive tract.