Zebrafish: An Emerging Model System for Human Disease and Drug Discovery

  title={Zebrafish: An Emerging Model System for Human Disease and Drug Discovery},
  author={G Rabe Kari and Ulrich Rodeck and Adam P. Dicker},
  journal={Clinical Pharmacology \& Therapeutics},
In vivo studies represent an essential step in drug development and currently rely largely on mice, yet limitations of mammalian models motivated the search for complementary vertebrate model systems. This review focuses on zebrafish, Danio rerio, as a facile model system to study human disease and drug responses. Zebrafish are particularly suited for this purpose because they represent a vertebrate species, their genome is sequenced, and a large number of synchronously developing, transparent… 

Zebrafish as a model system for biomedical studies

Genome organization and the pathways involved into control of signal transduction appear to be highly conserved between zebra fish and humans and therefore zebrafish may be used for modeling of human diseases.

Zebrafish xenografts as a tool for in vivo studies on human cancer

Current data on xenotransplantation of human cells into zebrafish is summarized, highlighting the advantages and limitations of this model in comparison to classical murine models of xenotranplantation.

Zebrafish as model organism for drug discovery and toxicity testing : A review

The zebrafish promises to contribute to several aspects of the drug developme nt process, including target identification, disease modelling, lead disc overy and toxicology, and the utility of this model in the drug discovery and toxicity studies is cited.

The zebrafish: a powerful platform for in vivo, HTS drug discovery.

The zebrafish (Danio rerio) is an emerging vertebrate model for drug discovery that permits whole animal drug screens with excellent throughput, combined with ease of use and low cost and provides new promise for the discovery of safe, specific, and powerful new drugs.

An Overview of Zebrafish Modeling Methods in Drug Discovery and Development.

Applying several various approaches such as using chemicals, pathogens, and genetic manipulation approaches allows zebrafish development into a preferable model that mimics some human disease pathophysiology and can pave the way for discovering new drugs that are accompanied by higher amelioration effects on different human diseases.

Zebrafish embryos and larvae: a new generation of disease models and drug screens.

It is argued that zebrafish embryos need to be precisely staged when used in acute assays, so as to ensure a consistent window of developmental exposure, and believes that z stripes embryo screens can be used in the pre-regulatory phases of drug development, although more validation studies are needed to overcome industry scepticism.

Zebrafish ( Danio Rerio ) Inmodeling Brain Disorders

This work highlights the use of Zebrafish in representing depression, brain tumor, epilepsy, and anxiety brain disorders by reviewing current studies and suggests new promises in defining disease pathway and discovering specific and powerful therapies.

Zebrafish model in drug safety assessment.

Although research on zebrafish as a model system began a few decades ago, later studies resulted in the characterization of a large number of genes involved in vertebrate development and biological pathways thus establishing zebra fish as a relevant human disease model for research.

Zebrafish Xenografts for the In Vivo Analysis of Healthy and Malignant Human Hematopoietic Cells.

A protocol for transplantation of healthy and malignant human hematopoietic cells into larval zebra fish is presented and possible advantages and limitations of the zebrafish compared to murine xenograft models are discussed.

Liver tumor models in transgenic zebrafish: an alternative in vivo approach to study hepatocarcinogenes.

Transgenic fusion of an oncogene and a color reporter, such as the green fluorescent protein, allows to conveniently monitor transgenic tumors for their initiation, progression, metastasis and transplantation in the transparent zebrafish embryos, as demonstrated with newly established liver cancer models.



In vivo drug discovery in the zebrafish

By combining the scale and throughput of in vitro screens with the physiological complexity of animal studies, the zebrafish promises to contribute to several aspects of the drug development process, including target identification, disease modelling, lead discovery and toxicology.

Rapid analysis of angiogenesis drugs in a live fluorescent zebrafish assay.

An assay using the zebrafish is described that provides the relevance of an in vivo environment as well as the potential for high throughput drug screening, and the ideal assay for screening new compounds should involve blood vessels growing in their natural environment, yet be amenable to rapid analysis.

Modeling Liver Cancer Using Zebrafish: A Comparative Oncogenomics Approach

Using comparative microarray analyses, comparative oncogenomic work provides a generalframework for comparing and validating microarray data of zebrafish model with human cancer, adding confidence of using the zebra fish to model human cancers.

Overview of the Zebrafish system.

Zebrafish: An Animal Model for Toxicological Studies

The versatile zebrafish is now incorporated in many areas of toxicological programs for assessing human risk and for preclinical drug discovery and screening.

In vivo imaging of embryonic vascular development using transgenic zebrafish.

It is found that the zebrafish fli1 promoter is able to drive expression of enhanced green fluorescent protein (EGFP) in all blood vessels throughout embryogenesis, and these transgenic lines allow detailed analysis of both wild type and mutant embryonic vasculature.

Zebrafish as a "biosensor"? Effects of ionizing radiation and amifostine on embryonic viability and development.

The results together suggest the power and versatility of the zebrafish in assessing the effects of radiation and radiomodifiers on organ and tissue development.