Zebrafish: An Emerging Model System for Human Disease and Drug Discovery

@article{Kari2007ZebrafishAE,
  title={Zebrafish: An Emerging Model System for Human Disease and Drug Discovery},
  author={G Rabe Kari and Ulrich Rodeck and Adam P. Dicker},
  journal={Clinical Pharmacology \& Therapeutics},
  year={2007},
  volume={82}
}
In vivo studies represent an essential step in drug development and currently rely largely on mice, yet limitations of mammalian models motivated the search for complementary vertebrate model systems. This review focuses on zebrafish, Danio rerio, as a facile model system to study human disease and drug responses. Zebrafish are particularly suited for this purpose because they represent a vertebrate species, their genome is sequenced, and a large number of synchronously developing, transparent… 

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References

SHOWING 1-10 OF 72 REFERENCES

In vivo drug discovery in the zebrafish

TLDR
By combining the scale and throughput of in vitro screens with the physiological complexity of animal studies, the zebrafish promises to contribute to several aspects of the drug development process, including target identification, disease modelling, lead discovery and toxicology.

Rapid analysis of angiogenesis drugs in a live fluorescent zebrafish assay.

TLDR
An assay using the zebrafish is described that provides the relevance of an in vivo environment as well as the potential for high throughput drug screening, and the ideal assay for screening new compounds should involve blood vessels growing in their natural environment, yet be amenable to rapid analysis.

Modeling Liver Cancer Using Zebrafish: A Comparative Oncogenomics Approach

TLDR
Using comparative microarray analyses, comparative oncogenomic work provides a generalframework for comparing and validating microarray data of zebrafish model with human cancer, adding confidence of using the zebra fish to model human cancers.

Overview of the Zebrafish system.

Zebrafish: An Animal Model for Toxicological Studies

TLDR
The versatile zebrafish is now incorporated in many areas of toxicological programs for assessing human risk and for preclinical drug discovery and screening.

In vivo imaging of embryonic vascular development using transgenic zebrafish.

TLDR
It is found that the zebrafish fli1 promoter is able to drive expression of enhanced green fluorescent protein (EGFP) in all blood vessels throughout embryogenesis, and these transgenic lines allow detailed analysis of both wild type and mutant embryonic vasculature.

Zebrafish as a "biosensor"? Effects of ionizing radiation and amifostine on embryonic viability and development.

TLDR
The results together suggest the power and versatility of the zebrafish in assessing the effects of radiation and radiomodifiers on organ and tissue development.
...