ZebraFISH: fluorescent in situ hybridization protocol and three-dimensional imaging of gene expression patterns.


We present a method and protocol for fluorescent in situ hybridization (FISH) in zebrafish embryos to enable three-dimensional imaging of patterns of gene expression using confocal laser scanning microscopy. We describe the development of our protocol and the processing workflow of the three-dimensional images from the confocal microscope. We refer to this… (More)
DOI: 10.1089/zeb.2006.3.465