ZccR--a MerR-like regulator from Bordetella pertussis which responds to zinc, cadmium, and cobalt.

@article{Kidd2003ZccRaMR,
  title={ZccR--a MerR-like regulator from Bordetella pertussis which responds to zinc, cadmium, and cobalt.},
  author={Stephen P Kidd and Nigel L. Brown},
  journal={Biochemical and biophysical research communications},
  year={2003},
  volume={302 4},
  pages={697-702}
}
A transcriptional regulator of the MerR family encoded by Bordetella pertussis was characterized in Escherichia coli and in vitro. Uniquely, the regulator responded specifically to Zn(II), Cd(II), and Co(II) and was named ZccR. Gel shift assays confirmed that ZccR binds to an adjacent divergent promoter possessing an elongated spacer region of 19bp between the -10 and -35 elements, and that Zn(II), Co(II), and Cd(II) reduced the protein affinity for DNA. Site-directed mutagenesis of four… CONTINUE READING