Z-DNA immunoreactivity of Drosophila polytene chromosomes. Effects of the fixatives 45% acetic acid and 95% ethanol and of DNase I nicking.


Drosophila salivary chromosomes have been isolated at neutral pH and physiological ionic strength. They display only background level binding of antibodies against Z-DNA. Following exposure to the commonly used fixative 45% acetic acid all of the polytene chromosomes, X and autosomes, show a massive increase in anti-Z-DNA antibody binding. The enhancement… (More)