• Corpus ID: 111374664

YscN,thePutative Energizer oftheYersinia Yop

  title={YscN,thePutative Energizer oftheYersinia Yop},
  author={Secretion Machinery and Guy Richard Cornelis},
motifs (boxes A andB)described byWalkeretal.(J.E. Walker, M.Saraste, M.J.Runswick, andN.J.Gay,EMBO J.1:945-951, 1982). We engineered a pYV mutant encoding amodified YscNprotein lacking boxA.Thismutant, impaired inYopsecretion, can becomplemented intransbya cloned yscNgene.We conclude thatYscNisa component oftheYopsecretion machinery using ATP.We hypothesize thatitiseither theenergizer ofthismachinery or a partofit. 


Secretion of Yop proteins by Yersiniae
The nucleotide sequence of genes yop20 and yop25 from Yersinia enterocolitica O:9 is presented, the first report of a yop 20 sequence of yersiniae, and evidences that Yops are not membrane proteins are presented.
Multiple sequence alignment withhierarchical clustering
An algorithm is presenteded for the multiple alignment of sequences, either proteins ornucleic acids, that is both accurate and easy to understand on microcompetence on microcomputing sites, based on the conventional dynamic-programming method of pairwise alignment.
LcrD, a membrane-bound regulator of the Yersinia pestis low-calcium response.
An lcrD mutant, in contrast to the parent Y. pestis, did not undergo growth restriction or induce strong expression of the V antigen when grown under conditions expected to elicit maximal expression of pCD1 genes.
The virulence protein Yop5 of Yersinia pseudotuberculosis is regulated at transcriptional level by plasmid‐plB1 ‐encoded trans‐acting elements controlled by temperature and calcium
The DNA sequence of the structural gene (yopE) of one of the Yersinia pseudotuberculosis virulence plas‐mid‐plB1‐encoded proteins, Yop5, is presented and a specific mutant, having a kanamycin‐resistance fragment inserted within the yopE gene was no longer virulent for mice.
A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
Certain environmental signals (i.e., osmolarity and the presence of amino acids) are tightly coupled to the expression of toxR-regulated proteins and therefore may be signals that are directly sensed by the ToxR protein.
Organization andEnvironmental Regulation ofthePseudomonas syringae pv.syringae 61hrpCluster
Results indicate that enhanced expression ofhrpgenesoccurs early intheinteraction with nonhost plant species in anapparent response toaltered nutritional conditions, and suggests that P.syringae strains mayrespond tonutritional conditions during theinduction stage.
Rapid and efficient site-specific mutagenesis without phenotypic selection.
  • T. Kunkel
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1985
The high efficiency, approximately equal to 10-fold greater than that observed using current methods without enrichment procedures, is obtained by using a DNA template containing several uracil residues in place of thymine, which is applied to mutations introduced via both oligonucleotides and error-prone polymerization.