Yield Enhancement Strategies for Enhancement of Indole Alkaloids in Hairy Root Cultures of Catharanthus Roseus

  title={Yield Enhancement Strategies for Enhancement of Indole Alkaloids in Hairy Root Cultures of Catharanthus Roseus},
  author={Dhara Thakore and Ashok K. Srivastava and Alok Krishna Sinha},
  journal={International Journal of Chemical Engineering and Applications},
Abstarct—Late exponential phase of hairy root cultures of Catharanthus roseus cultivated in a statistically optimized medium were treated with permeabilizing agents (DMSO, Triton X-100, n-hexadecane and Tween 80) to establish the effect of their concentrations and exposure time on the biomass and major alkaloid(ajmalicine) yield & productivity. The permeabilizing agents TritonX-100(0.1% v/v) and nhexadecane(2%v/v) led to 98% increase in the specific yield of ajmalicine in the roots. Statistical… 

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