Yeast transformation: a model system for the study of recombination.

  title={Yeast transformation: a model system for the study of recombination.},
  author={Terry L. Orr-Weaver and Jack W. Szostak and Rodney Rothstein},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={78 10},
DNA molecules that integrate into yeast chromosomes during yeast transformation do so by homologous recombination. We have studied the way in which circular and linear molecules recombine with homologous chromosomal sequences. We show that DNA ends are highly recombinogenic and interact directly with homologous sequences. Circular hybrid plasmids can integrate by a single reciprocal crossover, but only at a low frequency. Restriction enzyme digestion within a region homologous to yeast… 

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Methods in Yeast Genetics (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY)

  • Genetics
  • 1973