Yeast secretory expression of insulin precursors

@article{Kjeldsen2000YeastSE,
  title={Yeast secretory expression of insulin precursors},
  author={T. Kjeldsen},
  journal={Applied Microbiology and Biotechnology},
  year={2000},
  volume={54},
  pages={277-286}
}
  • T. Kjeldsen
  • Published 2000
  • Biology, Medicine
  • Applied Microbiology and Biotechnology
  • Abstract Since the 1980s, recombinant human insulin for the treatment of diabetes mellitus has been produced using either the yeast Saccharomyces cerevisiae or the prokaryote Escherichia coli. Here, development of the insulin secretory expression system in S. cerevisiae and its subsequent optimisation is described. Expression of proinsulin in S. cerevisiae does not result in efficient secretion of proinsulin or insulin. However, expression of a cDNA encoding a proinsulin-like molecule with… CONTINUE READING
    139 Citations
    Expression and Purification of C-Peptide Containing Insulin Using Pichia pastoris Expression System
    • 8
    • PDF
    Engineering of vesicle trafficking improves heterologous protein secretion in Saccharomyces cerevisiae.
    • 71
    Metabolic engineering of recombinant protein secretion by Saccharomyces cerevisiae.
    • 126
    • PDF
    Recombinant expression systems in the pharmaceutical industry
    • F. R. Schmidt
    • Biology, Medicine
    • Applied Microbiology and Biotechnology
    • 2004
    • 335