Yeast ribonuclease H(70) cleaves RNA-DNA junctions.

  title={Yeast ribonuclease H(70) cleaves RNA-DNA junctions.},
  author={R Karwan and Ulrike Wintersberger},
  journal={FEBS letters},
  volume={206 2},
A specific substrate, M13 DNA:RNA-[32P]DNA, was synthesized to investigate the mode of cleavage of enzymes with RNase H activity. RNase H(70) from Saccharomyces cerevisiae hydrolyzes the phosphodiester bond at the RNA-DNA junction of this substrate, thereby producing a 5'-monophosphate-terminated polydeoxyribonucleotide and 3'-hydroxyl-terminated… CONTINUE READING