The Retrograde Response and Other Pathways of Interorganelle Communication in Yeast Replicative Aging
The retrograde response is a prototypical pathway of interorganelle communication that lies at the nexus of metabolic regulation, stress resistance, chromatin-dependent gene regulation, and genome stability, and it is related to calorie restriction, which may be the result of the involvement of TOR.
The role of mitochondria in the aging processes of yeast.
- BiologySub-cellular biochemistry
The similarities and dissimilarities of the various aging processes of the most often used model organisms for aging research with respect to the mitochondrial theory of aging mitochondrial Theory of aging are given.
The integrated stress response in budding yeast lifespan extension
- BiologyMicrobial cell
It is demonstrated that yeast remains a powerful model system for elucidating conserved mechanisms to achieve lifespan extension that are likely to drive therapeutic approaches to extend human lifespan and healthspan.
Changes in Transcription and Metabolism During the Early Stage of Replicative Cellular Senescence in Budding Yeast*
- BiologyThe Journal of Biological Chemistry
Transcriptional and metabolic profiling for yeast at early stages of senescence (4th, 7th, and 11th generation), that is, for populations in which most cells are still alive, is performed, for the first integrated information on transcriptome and metabolome of aging yeast cells.
Aging Research in Yeast
- BiologySubcellular Biochemistry
The role of Mitochondria in the aging processes of yeast and the actin cytoskeleton is investigated, as well as genetic Approaches to Aging in Budding and Fission Yeasts: New Connections and New Opportunities.
Daughters of the budding yeast from old mothers have shorter replicative lifespans but not total lifespans. Are DNA damage and rDNA instability the factors that determine longevity?
- EngineeringCell cycle
It is shown for the first time that daughter cells of an old mother have a reset lifespan expressed in units of time despite drastic reduction of their budding lifespan, which suggests that a single yeast cell has a fixed programmed longevity regardless of the time point at which it was originated.
Budding yeast as a model organism to study the effects of age.
- BiologyFEMS microbiology reviews
The possible mechanisms allowing old cells to produce rejuvenated progeny are described in terms of accumulation and inheritance of aging factors and how changes in different organelles might interplay with each other are described.
Sir2 Blocks Extreme Life-Span Extension
Mitochondrial dysfunction, proteotoxicity, and aging: causes or effects, and the possible impact of NAD+-controlled protein glycation.
- BiologyAdvances in clinical chemistry
More than 10% of yeast genes are related to genome stability and influence cellular senescence via rDNA maintenance
- BiologyNucleic acids research
The link between rDNA state and senescence was broken after deletion of factors related with DNA polymerase ϵ, suggesting a model in which molecular events at the heart of the replication fork induce abnormal rDNA recombination and are responsible for the emergence of an aging signal.
Interorganelle signaling is a determinant of longevity in Saccharomyces cerevisiae.
The molecular mechanism of aging elucidated here parallels the results of genetic studies of aging in nematodes and fruit flies, as well as the caloric restriction paradigm in mammals, and it underscores the importance of metabolic regulation in aging, suggesting a general applicability.
Rtg2 protein links metabolism and genome stability in yeast longevity.
The results indicate that the suppression of ERC production by Rtg2p requires that it not be in the process of transducing the retrograde signal from the mitochondrion, and RTG2 lies at the nexus of cellular metabolism and genome stability, coordinating two pathways that have opposite effects on yeast longevity.
Metabolic Control and Gene Dysregulation in Yeast Aging
- BiologyAnnals of the New York Academy of Sciences
It is now clear that the functional status of chromatin plays an important role in aging, and an optimal level of Ras2p activity is required for maximum longevity.
Cellular responses to mitochondrial dysfunction: it's not always downhill
- BiologyCell Death and Differentiation
In yeast cells with dysfunctional mitochondria, major rearrangements of carbohydrate and nitrogen metabolism take place through pathways of intracellular signaling from mitochondria to the nucleus ± a response called retrograde regulation, which is not restricted to yeast cells, however.
Aged mother cells of Saccharomyces cerevisiae show markers of oxidative stress and apoptosis
- BiologyMolecular microbiology
It is shown by staining with dihydrorhodamine that old yeast mother cells isolated by elutriation, but not young cells, contain ROS that are localized in the mitochondria, pointing to a role for oxygen in the yeast ageing process.
Prohibitins and Ras2 protein cooperate in the maintenance of mitochondrial function during yeast aging.
- BiologyActa biochimica Polonica
It is demonstrated that the elimination of one or both of the prohibitin genes in yeast markedly diminished the replicative life span of cells that lack fully-functional mitochondria, while having no effect on cells with functioning mitochondria.
Passage through stationary phase advances replicative aging in Saccharomyces cerevisiae.
- BiologyProceedings of the National Academy of Sciences of the United States of America
It is reported here that cells starved for nutrients during prolonged periods in stationary phase show a decrease in generational lifespan when they reenter the cell cycle, suggesting that Stationary-phase yeast cells may be a useful system for identifying factors that affect aging in other nondividing eukaryotic cells.
Genome-wide responses to mitochondrial dysfunction.
- BiologyMolecular biology of the cell
It is shown that respiratory deficiency, but not inhibition of mitochondrial ATP synthesis per se, induces a suite of genes associated with both peroxisomal activities and metabolite-restoration pathways that would mitigate the loss of a complete tricarboxylic acid cycle.
Chronological aging leads to apoptosis in yeast
- BiologyThe Journal of cell biology
It is suggested that apoptosis in yeast confers a selective advantage for this unicellular organism, and that old yeast cells release substances into the medium that stimulate survival of the clone.
Divergent roles of RAS1 and RAS2 in yeast longevity.
- BiologyThe Journal of biological chemistry
The results suggest that RAS1 and RAS2 play reciprocal roles in determining yeast longevity, and that the cAMP pathway is neither sufficient nor necessary for increased longevity.