Yeast polypeptide fusion surface display levels predict thermal stability and soluble secretion efficiency.

@article{Shusta1999YeastPF,
  title={Yeast polypeptide fusion surface display levels predict thermal stability and soluble secretion efficiency.},
  author={Eric V Shusta and Michele C. Kieke and Elyse Parke and David M. Kranz and K Dane Wittrup},
  journal={Journal of molecular biology},
  year={1999},
  volume={292 5},
  pages={949-56}
}
Efficiency of yeast cell surface display can serve as a proxy screening variable for enhanced thermal stability and soluble secretion efficiency of mutant proteins. Several single-chain T cell receptor (scTCR) single-site mutants that enable yeast surface display, along with their double and triple mutant combinations, were analyzed for soluble secretion from the yeast Saccharomyces cerevisiae. While secretion of the wild-type scTCR was not detected, each of the single, double, and triple… CONTINUE READING

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