Yeast histone H4 N-terminal sequence is required for promoter activation in vivo

  title={Yeast histone H4 N-terminal sequence is required for promoter activation in vivo},
  author={L. Durrin and Randall K. Mann and P. Kayne and M. Grunstein},
  • L. Durrin, Randall K. Mann, +1 author M. Grunstein
  • Published 1991
  • Biology, Medicine
  • Cell
  • To search for histone domains that may regulate transcription in vivo, we made deletions and amino acid substitutions in the histone N-termini of S. cerevisiae. Histone H4 N-terminal residues 4-23, which include the extremely conserved, reversibly acetylated lysines (at positions 5, 8, 12, and 16), were found to encompass a region required for the activation of the GAL1 promoter. Deletions in the H4 N-terminus reduce GAL1 activation 20-fold. This effect is specific to histone H4 in that large… CONTINUE READING
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