Yeast expression and NMR analysis of the extracellular domain of muscle nicotinic acetylcholine receptor alpha subunit.

@article{Yao2002YeastEA,
  title={Yeast expression and NMR analysis of the extracellular domain of muscle nicotinic acetylcholine receptor alpha subunit.},
  author={Yun Yao and Junmei Wang and Nitnara Viroonchatapan and Avraham O Samson and Jordan H Chill and Elizabeth E Rothe and Jacob A Anglister and Z W Wang},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 15},
  pages={12613-21}
}
The alpha subunit of the nicotinic acetylcholine receptor (AChR) from Torpedo electric organ and mammalian muscle contains high affinity binding sites for alpha-bungarotoxin and for autoimmune antibodies in sera of patients with myasthenia gravis. To obtain sufficient materials for structural studies of the receptor-ligand complexes, we have expressed part of the mouse muscle alpha subunit as a soluble, secretory protein using the yeast Pichia pastoris. By testing a series of truncated… CONTINUE READING

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