Yeast Tdp1 regulates the fidelity of nonhomologous end joining

  title={Yeast Tdp1 regulates the fidelity of nonhomologous end joining},
  author={Karim Bahmed and K. C. Nitiss and J. Nitiss},
  journal={Proceedings of the National Academy of Sciences},
  pages={4057 - 4062}
  • Karim Bahmed, K. C. Nitiss, J. Nitiss
  • Published 2010
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences
  • Tyrosyl-DNA-phosphodiesterase 1 (Tdp1) can disjoin peptides covalently bound to DNA. We assessed the role of Tdp1 in nonhomologous end joining (NHEJ) and found that linear DNA molecules with 5′ extensions showed a high frequency of misrepair in Δtdp1 cells. The joining errors in Δtdp1 cells were predominantly 2-4 nucleotide insertions. Ends with 3′ extensions or blunt ends did not show enhanced frequencies of errors, although Δtdp1 cells repaired blunt DNA ends with greater efficiency than WT… CONTINUE READING
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