Yeast Tdp1 regulates the fidelity of nonhomologous end joining.

@article{Bahmed2010YeastTR,
  title={Yeast Tdp1 regulates the fidelity of nonhomologous end joining.},
  author={Karim Bahmed and Karin C. Nitiss and John L Nitiss},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2010},
  volume={107 9},
  pages={
          4057-62
        }
}
Tyrosyl-DNA-phosphodiesterase 1 (Tdp1) can disjoin peptides covalently bound to DNA. We assessed the role of Tdp1 in nonhomologous end joining (NHEJ) and found that linear DNA molecules with 5' extensions showed a high frequency of misrepair in Deltatdp1 cells. The joining errors in Deltatdp1 cells were predominantly 2-4 nucleotide insertions. Ends with 3' extensions or blunt ends did not show enhanced frequencies of errors, although Deltatdp1 cells repaired blunt DNA ends with greater… CONTINUE READING

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