[Yeast (Saccharomyces cerevisiae) secretory expression vector maintained stably in Pro3+ transformants in rich medium].

Abstract

A yeast (Saccharomyces cerevisiae) secretory expression vector containing PRO3 gene was constructed (pCBy310). beta HCG(Human choriogonadotropin beta subunit)-cDNA was inserted into pCBy310 to form a recombinant plasmid pCBy314. Since yeast proline auxotroph will not survive in rich medium (YPD), YPD could be used as a selection pressure, and pCBy314 could… (More)

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@article{Xie2000YeastC, title={[Yeast (Saccharomyces cerevisiae) secretory expression vector maintained stably in Pro3+ transformants in rich medium].}, author={Heng Yue Xie and Y Tang and W. D. Jiang and Haiyan He and Mingyuan Liu and Darren Kuang}, journal={Yi chuan xue bao = Acta genetica Sinica}, year={2000}, volume={27 4}, pages={369-76} }