Yeast RAD3 protein binds directly to both SSL2 and SSL1 proteins: implications for the structure and function of transcription/repair factor b.

@article{Bardwell1994YeastRP,
  title={Yeast RAD3 protein binds directly to both SSL2 and SSL1 proteins: implications for the structure and function of transcription/repair factor b.},
  author={Lee Bardwell and A. Jane Bardwell and William J. Feaver and Jesper Qualmann Svejstrup and Roger D. Kornberg and Errol C. Friedberg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1994},
  volume={91 9},
  pages={3926-30}
}
The RAD3 and SSL2 gene products are essential proteins that are also required for the nucleotide excision repair pathway. We have recently demonstrated that the RAD3 gene product along with the SSL1 and TFB1 gene products are subunits of RNA polymerase II basal transcription factor b. Additionally, the SSL2 gene product physically interacts with purified factor b. Here we combine an in vitro immunoprecipitation assay and an in vivo genetic assay to demonstrate a series of pairwise protein… CONTINUE READING