Yeast Pre-rRNA Processing and Modification Occur Cotranscriptionally

@inproceedings{Ko2010YeastPP,
  title={Yeast Pre-rRNA Processing and Modification Occur Cotranscriptionally},
  author={Martin Ko{\vs} and David Tollervey},
  booktitle={Molecular cell},
  year={2010}
}
To better understand yeast ribosome synthesis, we developed techniques for the rapid harvesting and analysis of metabolically labeled cultures. Modeling of the resulting kinetic data allowed predicted lifetimes and processing patterns to be compared with the experimental data. This supported a transcription time for the 35S primary transcripts of approximately 170 s at 30 degrees C (approximately 40 nt s(-1)), with a high fraction (approximately 70%) of nascent transcripts cleaved at the early… CONTINUE READING

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