Yeast Expression Systems: Overview and Recent Advances

@article{Baghban2019YeastES,
  title={Yeast Expression Systems: Overview and Recent Advances},
  author={Roghayyeh Baghban and Safar Farajnia and Masoumeh Rajabibazl and Younes Ghasemi and Amirali Mafi and Reyhaneh Hoseinpoor and Leila Rahbarnia and Maryam Aria},
  journal={Molecular Biotechnology},
  year={2019},
  volume={61},
  pages={365-384}
}
Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications. Great attention has been emerged on yeast due to the inherent advantages and new developments in this host cell. For the production of each specific product, the most appropriate expression system should be identified and optimized both on the genetic and fermentation levels, considering the features of the host, vector and expression strategies. Currently, several new… 
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References

SHOWING 1-10 OF 252 REFERENCES
Production of recombinant proteins by yeast cells.
Non-conventional yeasts as hosts for heterologous protein production.
TLDR
The current status of Kluyveromyces lactis, Yarrowia lipolytica, Hansenula polymorpha and Pichia pastoris (the best-known alternative yeast systems) is reviewed and the advantages and limitations of these systems are discussed in relation to S. cerevisiae.
Heterologous Protein Expression and Secretion in Yarrowia lipolytica
TLDR
Yarrowia’s characteristics regarding heterologous protein production are presented, recent developments of the Yarrowia expression system, such as surface display vectors, engineered strains and high-throughput screening processes, will be reviewed.
Optimising yeast as a host for recombinant protein production (review).
TLDR
An overview of the opportunities available to improve yeast as a host system for recombinant protein production is provided and molecular mechanisms and culture parameters underpinning high yields are identified.
Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae
TLDR
For the IP there is more than 10‐fold higher protein production with the CPOTud vector compared with the standard URA3‐based vector, and this vector system therefore represent a valuable resource for future studies and optimization of recombinant protein production in yeast.
New Developments in Pichia pastoris Expression System, Review and Update.
TLDR
New developments that occurred in the P. pastoris expression system converted this system to a versatile host for the production of complex proteins, paving the way for several proteins to enter the clinical trials or industrial processes with this valuable expression host.
Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production
TLDR
The purpose of this review is to summarize important developments and features of this expression system and to examine from an experimental perspective the genetic engineering, protein chemical and molecular design considerations that have to be taken into account for the successful expression of the target recombinant protein.
...
...