Yeast DNA ligase IV mediates non-homologous DNA end joining

@article{Wilson1997YeastDL,
  title={Yeast DNA ligase IV mediates non-homologous DNA end joining},
  author={Thomas E. Wilson and Ulf Grawunder and Michael R Lieber},
  journal={Nature},
  year={1997},
  volume={388},
  pages={495-498}
}
The discovery of homologues from the yeast Saccharomyces cerevisiae of the human Ku DNA-end-binding proteins (HDF1 and KU80) has established that this organism is capable of non-homologous double-strand end joining (NHEJ), a form of DNA double-strand break repair (DSBR) active in mammalian V(D)J recombination. Identification of the DNA ligase that mediates NHEJ in yeast will help elucidate the function of the four mammalian DNA ligases in DSBR, V(D)J recombination and other reactions. Here we… 

Saccharomyces cerevisiae DNA Ligase IV Supports Imprecise End Joining Independently of Its Catalytic Activity

Findings indicate that Dnl4 can promote mutagenic end joining independently of its catalytic activity, likely by a mechanism that involves Cdc9.

Identification of genes involved in repair of DNA double-strand breaks in mammalian cells.

The evidence implicating four proteins as functioning in NHEJ is reviewed and their properties and role in other pathways are discussed and the significance of DSB repair to clinical radiosensitivity and human disorders is evaluated.

Completion of base excision repair by mammalian DNA ligases.

Saccharomyces cerevisiae LIF 1 : a function involved in DNA double-strand break repair related to mammalian XRCC 4

The discovery of LIF1, a S.cerevisiae protein that strongly interacts with the C-terminal BRCT domain of yeast LIG4, allows for mutational analyses of structure–function relationships in XRCC4-like proteins to define their role in DNA double-strand break repair.

Arabidopsis DNA ligase IV is induced by gamma-irradiation and interacts with an Arabidopsis homologue of the double strand break repair protein XRCC4.

An Arabidopsis thaliana homologue (AtLIG4) of human and S. cerevisiaeDNA ligase IV is identified which is shown to encode an ATP-dependent DNA ligase with a theoretical molecular mass of 138 kDa and 48% similarity in amino-acid sequence to the human DNA ligasing protein XRCC4.

Genetic evidence for the involvement of DNA ligase IV in the DNA-PK-dependent pathway of non-homologous end joining in mammalian cells.

End joining in vitro is dominated by pathways with properties similar to B-NHEJ that do not display a strong dependence on DNA ligase IV, with D-N HEJ retaining only a limited contribution.

Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism

Interactions of the DNA ligase IV-associated proteins Lif1p and XRCC4 of yeast and human with the putatively orthologous G-patch proteins Ntr1p/Spp382p and NTR1/TFIP11 that have recently been implicated in mRNA splicing are described.
...

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